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	Provedor de dados ArchiMer (1) Autor Chatain, Beatrice (1) Daule, Sophie (1) Grima, Laure (1) Guinand, Bruno (1) Vandeputte, Marc (1) Vergnet, Alain (1) Mais... Palavra-chave Dicentrarchus labrax (1) European sea bass (1) Fasting tolerance (1) Feed efficiency (1) Selective breeding (1) Tipo do documento Text (1) Ano 2014 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 181 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última Identification of duplicates of cassava accessions sampled on the North Region of Brazil using microsatellite markers Acta Amazonica Moura,Elisa Ferreira; Farias Neto,João Tomé de; Sampaio,José Edson; Silva,Diehgo Tuloza da; Ramalho,Girena Fernandes. Duplicates are common in germplasm banks and their identification is needed to facilitate germplasm bank management and to reduce maintenance costs. The aim of this work was to identify duplicates of cassava from a germplasm bank in Eastern Amazon, which had been previously characterized both morphological and agronomically. In order to be genotyped with 15 microsatellite loci, 36 accessions were selected. These accessions were classified into 13 groups of similar morpho-agronomical characteristics. All loci were polymorphic, and 75 alleles were identified, with an average of five alleles per loci and H E = 0.66. There were determined 34 pairs of genotypes with identical multiloci profiles and the probability of genetic identity was 1.1x10-12 with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; Germplasm; Molecular markers. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672013000400008 Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers Electron. J. Biotechnol. Vanzetti,Leonardo Sebastián; Campos,Pablo; Demichelis,Melina; Lombardo,Lucio Andres; Aurelia,Paola Romina; Vaschetto,Luis María; Bainotti,Carlos Tomás; Helguera,Marcelo. Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust... Tipo: Journal article Palavras-chave: Genetic control of pathogens; Molecular markers; Plant breeding. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300009 Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers Ciência e Agrotecnologia Emara,Hassan Mahmoud; Omar,Ayman Feisal; El-Shamy,Moustafa Mahmoud; Mohamed,Mona Elsaid. ABSTRACT Powdery mildew of wheat (Triticum spp.) caused by Blumeria graminis f.sp. tritici (DC) E.O. Speer Em. Marchal is one of the most important bread wheat diseases in Egypt. All the Egyptian common bread wheat cultivars are susceptible to that disease at seedling and adult stages. Breeding of resistant cultivars is the most economical and environmentally safe method to eliminate the disease and reduce crop losses. Combinations of two or more effective resistance genes may lead to better, more durable resistance to that disease. Eight Pm genes i.e. Pm2, Pm6, Pm12, Pm16, Pm24, Pm35, Pm36 and Pm37 out of 21 powdery mildew monogenic wheat lines (Pm) were resistant to 42 individual isolates of powdery mildew collected from different governorates in the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Powdery mildew; Genetic control; DNA; Molecular markers. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000300279 Identification of rapd marker linked to blast resistance gene in a somaclone of rice cultivar Araguaia Trop. Plant Pathol. ARAÚJO,LEILA G.; PRABHU,ANNE S.; FILIPPI,MARTA C.. The gene Pi-ar confers resistance to Pyricularia grisea race IB-45 in a somaclone derived from immature panicles of the susceptible rice (Oryza sativa) cultivar Araguaia. RAPD technique was used to identify molecular markers linked to this gene utilizing bulked segregant analysis. Initially, the two parental DNAs from the resistant donor SC09 and 'Araguaia' were analyzed using random primers. Of the 240 primers tested, 203 produced amplification products. The two parental DNAs along with the resistant and susceptible bulks of F2 population were screened using 48 primers that differentiated resistant and susceptible parents. Even though eight primers differentiated the resistant bulk from the susceptible bulk, as well as somaclone SC09 and 'Araguaia', only... Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Disease resistance; Somaclonal variation; Pyricularia grisea. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200010 Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars PAB Ochoa,Elisa del Carmen Martínez; Andrade-Rodríguez,María; Rodríguez,Mario Rocandio; Monter,Angel Villegas. The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Embryo culture; Funiculus; Molecular markers; Polymorphism. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010 Laboratory Protocols CIMMYT Applied Molecular Genetics Laboratory AgEcon Warburton, Marilyn. Third Edition Tipo: Report Palavras-chave: Chemiluminescence immunoassays; DNA; DNA hybridization; Molecular markers; PCR; RFLPs; Research and Development/Tech Change/Emerging Technologies; F30. Ano: 2005 URL: http://purl.umn.edu/56184 Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers Genet. Mol. Biol. Briñez,Boris; Perseguini,Juliana Morini Küpper Cardoso; Rosa,Juliana Santa; Bassi,Denis; Gonçalves,João Guilherme Ribeiro; Almeida,Caléo; Paulino,Jean Fausto de Carvalho; Blair,Matthew Ward; Chioratto,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Valdisser,Paula Arielle Mendes Ribeiro; Vianello,Rosana Pereira; Benchimol-Reis,Luciana Lasry. Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all... Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Interpopulation gene-pool; Molecular markers; QTL mapping; Water deficit. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500813 Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries Genet. Mol. Biol. Molina,Lorrayne Gomes; Cordenonsi da Fonseca,Guilherme; Morais,Guilherme Loss de; de Oliveira,Luiz Felipe Valter; Carvalho,Joseane Biso de; Kulcheski,Franceli Rodrigues; Margis,Rogerio. A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Next generation sequencing; Small RNA; SiRNA; Molecular markers. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200010 Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil Scientia Agricola Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Lopera-Barrero,Nelson Mauricio; Jacometo,Carolina Bespalhok; Vargas,Lauro; Gomes,Patrícia Cristina; Lopes,Taís da Silva. Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O: 0.628) higher than the broodstock A (alleles: 3.5 and H O: 0.600). Alleles with low frequency levels were observed in the both broodstocks. The positive coefficients of endogamy in the locus Pme2 (broodstock A: F IS = 0.30 and broodstock B: F IS = 0.20), Pme5... Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Genetic variability; Hatchery stations; Molecular markers. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300006 Microsatellite loci development for three catfish species from northwestern South America Neotropical Ichthyology Restrepo-Escobar,Natalia; Márquez,Edna J.. ABSTRACT The Neotropical catfish species Ageneiosus pardalis, Pimelodus grosskopfii, and Sorubim cuspicaudus are important fishery resources in Colombia that show historical declines in their capture. This study used next-generation sequencing with 454 FLX technology (Roche Applied Science) and bioinformatics analysis to develop between 18 and 24 microsatellite loci for these species. The novel microsatellite loci showed high values of polymorphic information content -PIC (A. pardalis: 0.601-0.903, P. grosskopfii: 0.748-0.946 and S. cuspicaudus: 0.383-0.876), and the average number of alleles/locus ranged from 7-15 for A. pardalis, 9-30 for P. grosskopfii and 5-14 for S. cuspicaudus. The average observed and expected heterozygosities were respectively,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fish; Molecular markers; Next-generation sequencing; Siluriformes. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100210 Microsatellite markers for identification of a group of italian olive accessions Scientia Agricola Muzzalupo,Innocenzo; Stefanizzi,Francesca; Salimonti,Amelia; Falabella,Rosanna; Perri,Enzo. Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars. Tipo: Info:eu-repo/semantics/article Palavras-chave: Olea europaea; Fingerprinting; Genetic identity; Molecular markers. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500014 Microsatellites for genetic studies and breeding programs in common bean PAB Campos,Tatiana de; Benchimol,Luciana Lasry; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de. Twenty microsatelitte loci were identified and characterized in common bean. Microsatellites were tested in 14 genotypes. The allele number ranged from 1 to 3, and the polymorphism information content (PIC) was between 0.14 and 0.65. These polymorphic markers are available to be used for breeding programs. Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; SSR; Molecular markers. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400018 Molecular and morphological data from Thoosidae in favour of the creation of a new suborder of Tetractinellida ArchiMer Luis Carballo, Jose; Bautista-guerrero, Eric; Cardenas, Paco; Cruz-barraza, Jose Antonio; Aguilar-camacho, Jose Maria. The Thoosidae (Porifera, Demospongiae, Tetractinellida) currently includes the genera Thoosa, Alectona, and Delectona. To this date, molecular data are only available for Alectona. In this study, the phylogenetic affinities of the genera Thoosa and Alectona have been investigated with the species T. mismalolli, T. calpulli, and T. purpurea from the Mexican Pacific using morphology and three molecular loci: the mitochondrial cytochrome oxidase subunit 1 (CO1 mtDNA), 28S rRNA (fragment D2), and 18S rRNA. Morphology and embryology showed that these genera are quite different from the rest of the tetractinellids because larvae of Alectona and Thoosa have unique features in sponges, such as the presence of monaxonic discs in Thoosa and tetraxonic discs in... Tipo: Text Palavras-chave: Astrophorina; Molecular markers; Porifera; Systematics; Synapomorphy; Thoosidae. Ano: 2018 URL: https://archimer.ifremer.fr/doc/00688/79996/83503.pdf Molecular characterisation and similarity relationships among iranian basil (Ocimum basilicum L.) accessions using inter simple sequence repeat markers Rev. Ciênc. Agron. Aghaei,Mohammad; Darvishzadeh,Reza; Hassani,Abbas. The study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. In the present study, genetic diversity among 50 Iranian basil (Ocimum basilicum L.) accessions was determined using inter simple sequence repeat (ISSR) markers. Thirty-eight alleles were generated at 12 ISSR loci. The number of alleles per locus ranged from 1 to 5 with an average of 3.17. The maximum number of alleles was observed at the A7, 818, 825 and 849 loci, and their size ranged from 300 to 2500 bp. A similarity matrix based on Jaccard's coefficient for all 50 basil accessions gave values from 1.00-0.60. The maximum similarity (1.00) was observed between the "Urmia" and "Shahr-e-Rey II" accessions as well as between... Tipo: Info:eu-repo/semantics/article Palavras-chave: Basil; Cluster analysis; Molecular markers; Plant genetic diversity. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000200014 Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers BABT Motta,Ludymila Brandão; Soares,Taís Cristina Bastos; Ferrão,Maria Amélia Gava; Caixeta,Eveline Teixeira; Lorenzoni,Rodrigo Monte; Souza Neto,José Dias de. The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea Arabica; Coffea canephora; Genetic diversity; Molecular markers. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500728 Molecular characterization of Brazilian Dicyma pulvinata isolates Trop. Plant Pathol. Tavares,Eder T.; Tigano,Myrian S.; Mello,Sueli C. M.; Martins,Irene; Cordeiro,Celia M. T.. Forty-nine Brazilian Dicyma pulvinata isolates were examined by morphological traits and RFLP, RAPD and AFLP analyses. This fungus is a mycoparasite of Microcyclus ulei, the causal agent of the most devastating rubber (Hevea brasiliensis) disease, known as "South American Leaf Blight" (SALB). These isolates were compared with an Indian isolate from Cercosporidium sp., and a French isolate from Cladosporium fulvum. They were also compared with Dicyma ampullifera from Papua New Guinea. The morphological parameters analyzed confirmed the identification of the Brazilian isolates. The graphic representations of the distance matrices of each molecular marker showed similar results. Dicyma pulvinata isolates from M. ulei were closely related, whereas the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Molecular markers; Biological control. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200005 Molecular characterization of elephantgrass accessions through RAPD markers Ciência e Agrotecnologia Passos,Leônidas Paixão; Machado,Marco Antonio; Vidigal,Maria Coletta; Campos,Ana Lúcia. Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average... Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Elephantgrass; RAPD; DNA. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009 Molecular characterization of grapevine from Santa Catarina, Brazil, using microsatellite markers PAB Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da. The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008 Molecular description and similarity relationships among native germplasm potatoes (Solanum tuberosum ssp. tuberosum L.) using morphological data and AFLP markers Electron. J. Biotechnol. Solano Solis,Jaime; Morales Ulloa,Daniza; Anabalón Rodríguez,Leonardo. Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A... Tipo: Journal article Palavras-chave: Cluster analysis; Molecular markers; Solanum tuberosum ssp. tuberosum. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300011 Molecular identification of intergenus crosses involving catfish hybrids: risks for aquaculture production Neotropical Ichthyology Hashimoto,Diogo T.; Prado,Fernanda D.; Foresti,Fausto; Porto-Foresti,Fábio. ABSTRACT Monitoring of the interspecific hybrid production and trade is essential for the appropriate management of these animals in fish farms. The identification of catfish hybrids by morphological analysis is unreliable, particularly of juveniles and post-F1 individuals. Therefore, in the present study, we used five molecular markers (four nuclear genes and one mitochondrial gene) to detect hybrids in the trade of pimelodid juvenile fish from different stocks purchased of five seed producers in Brazil. Samples commercialized as pintado (pure species Pseudoplatystoma corruscans ) from three fish farms were genetically identified as hybrid cachapinta (♀ P. reticulatum x ♂ P. corruscans ). In the stocks purchased as cachandiá (hybrid between ♀ P.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish farm; Hybridization; Molecular markers; Nuclear genes; Pimelodidae. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200213 Registros recuperados: 181 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última

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